Anti-TRIB2 Antibody

Western blot analysis of TRIB2 expression in Jurkat (A), MCF7 (B), SW480 (C), A375 (D) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 50 kD)
Immunofluorescent analysis of TRIB2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Jurkat cells using Anti-TRIB2 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF/IC
Primary Accession	Q92519
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	38801 Da

Additional Information
Gene ID	28951
Other Names	TRB2; Tribbles homolog 2; TRB-2
Target/Specificity	Recombinant fusion protein of human TRIB2 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) FC~~FC (1/100 - 1/200) IF/IC~~N/A
Format	Mouse IgG2a. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TRIB2 (HGNC:30809)
Function	Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity (By similarity).
Cellular Location	Cytoplasm. Cytoplasm, cytoskeleton. Note=May associate with the cytoskeleton.
Tissue Location	Highly expressed in peripheral blood leukocytes.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.