Anti-TRIM29 Antibody

Western blot analysis of TRIM29 expression in Hela (A), HepG2 (B), LOVO (C), A431 (D) whole cell lysates. (Predicted band size: 66 kD; Observed band size: 70 kD)
Immunofluorescent analysis of TRIM29 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of HL60 cells using Anti-TRIM29 Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF/IC
Primary Accession	Q14134
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	65835 Da

Additional Information
Gene ID	23650
Other Names	ATDC; Tripartite motif-containing protein 29; Ataxia telangiectasia group D-associated protein
Target/Specificity	Recombinant fusion protein of human TRIM29 expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) FC~~FC (1/100 - 1/200) IF/IC~~N/A
Format	Mouse IgG2a. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TRIM29
Synonyms	ATDC
Function	Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation.
Cellular Location	Cytoplasm. Lysosome. Note=Colocalizes with intermediate filaments
Tissue Location	Expressed in placenta, prostate and thymus.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.