Anti-Vimentin Antibody

Western blot analysis of Vimentin expression in Jurkat (A), K562 (B), SKNSH (C), SHSY5Y (D), Hela (E), NIH/3T3 (F), C6 (G), RAW264.7 (H) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 53 kD)
Immunohistochemical analysis of Vimentin staining in human cervical cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow cytometric analysis of HeLa cells using Anti-Vimentin Antibody (green) and negative control (red).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, FC
Primary Accession	P08670
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	53652 Da

Additional Information
Gene ID	7431
Other Names	Vimentin
Target/Specificity	Recombinant fusion protein of human Vimentin expressed in E. Coli
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 FC~~FC (1/100 - 1/200)
Format	Mouse IgG2a. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	VIM (HGNC:12692)
Function	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front (By similarity). Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner (By similarity). May promote axon outgrowth and motor fiber repair via DSP- mediated recruitment to outgrowth tips (By similarity).
Cellular Location	Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix {ECO:0000250\|UniProtKB:P31000}. Cell membrane {ECO:0000250\|UniProtKB:P20152}. Cell projection, axon {ECO:0000250\|UniProtKB:P20152}
Tissue Location	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.