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CD3E Mouse mAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | P22646 |
| Other Accession | P22646 |
| Reactivity | Rat, Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Calculated MW | 21393 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from mouse CD3E |
| Isotype | IgG |
| Purity | affinity purified by Protein G |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Membrane; Single-pass type I membrane protein. |
| SIMILARITY | Contains 1 Ig-like (immunoglobulin-like) domain.Contains 1 ITAM domain. |
| SUBUNIT | he TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2. |
| Gene ID | 12501 |
|---|---|
| Other Names | CD3epsilon; IMD18; T3E; TCRE; CD3; CD3E_HUMAN; CD3E; T-cell surface antigen T3/Leu-4 epsilon chain; CD3E_MOUSE; CD3 epsilon subunit of T-cell receptor complex; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e molecule; T-cell surface glycoprotein CD3 epsilon chain |
| Dilution | WB=1:1000-2000,IHC-P=1:200-800,IHC-F=1:200-800,IF=1:200-800 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Cd3e |
|---|---|
| Function | Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response (PubMed:9843989, PubMed:10952314, PubMed:19956738, PubMed:24470497, PubMed:24899501, PubMed:40592325). When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z (PubMed:10952314, PubMed:19956738, PubMed:24899501, PubMed:40592325). All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain (PubMed:10952314, PubMed:19956738, PubMed:24899501, PubMed:40592325). Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:10952314, PubMed:19956738, PubMed:24899501, PubMed:40592325). CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:10952314). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:10952314, PubMed:19956738, PubMed:24899501). Also participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (By similarity). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1 (By similarity). Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P07766} |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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