Bif Rabbit pAb

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Predicted	Rat, Pig, Human, Mouse, Dog, Horse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	40 KDa
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human Bif
Epitope Specificity	151-250/365
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasm. Golgi apparatus membrane. Mitochondrion outer membrane. Association with the Golgi apparatus depends on the cell type.
SIMILARITY	Belongs to the endophilin family. Contains 1 BAR domain. Contains 1 SH3 domain.
SUBUNIT	Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1 (By similarity). Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding.
Post-translational modifications	Phosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Tissue specificity: Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.

Additional Information
Gene ID	51100
Other Names	Bif-1; CGI-61; PPP1R70; dJ612B15.2; SH3 domain containing GRB2 like, endophilin B1; SH3-domain, GRB2-like, endophilin B1; protein phosphatase 1, regulatory subunit 70
Target/Specificity	Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Name	Bif Rabbit pAb
Function	May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion.