CCDC47 Rabbit pAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	Q96A33
Other Accession	Q96A33
Reactivity	Mouse
Predicted	Rat, Pig, Human, Rabbit, Chicken, Dog, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	55874 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human CCDC47
Epitope Specificity	375-483/483
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Additional Information
Gene ID	57003
Other Names	GK001; MSTP041; THNS; 2610204L23Rik; asp4; calumin; RGD1308813; CCD47_HUMAN; CCDC47; Coiled-coil domain-containing protein 47; CCD47_MOUSE; Adipocyte-specific protein 4; CCD47_RAT; coiled-coil domain containing 47
Dilution	WB=1:500-2000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CCDC47 {ECO:0000303\|PubMed:30401460, ECO:0000312\|HGNC:HGNC:24856}
Function	Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed:32814900, PubMed:32820719, PubMed:36261522). The MPT complex takes over after the SEC61 complex: following membrane insertion of the first few transmembrane segments of proteins by the SEC61 complex, the MPT complex occludes the lateral gate of the SEC61 complex to promote insertion of subsequent transmembrane regions (PubMed:36261522). Within the MPT complex, the PAT subcomplex sequesters any highly polar regions in the transmembrane domains away from the non-polar membrane environment until they can be buried in the interior of the fully assembled protein (By similarity). Within the PAT subcomplex, CCDC47 occludes the lateral gate of the SEC61 complex (By similarity). Involved in the regulation of calcium ion homeostasis in the ER (PubMed:30401460). Required for proper protein degradation via the ERAD (ER-associated degradation) pathway (PubMed:25009997). Has an essential role in the maintenance of ER organization during embryogenesis (By similarity).
Cellular Location	Endoplasmic reticulum membrane; Single-pass type I membrane protein. Rough endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:Q9D024}; Single-pass type I membrane protein