SLK Rabbit pAb

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Predicted	Rat, Human, Mouse, Rabbit, Dog, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	143 KDa
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SLK
Epitope Specificity	885-931/1235
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain. Contains 1 UVR domain.
Post-translational modifications	Proteolytically cleaved by caspase-3. Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	SLK is a member of the serine/threonine kinase subfamily, Ste20. This subfamily is comprised of several mammalian kinases which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase Ste20, a protein involved in relaying signals from G protein-coupled receptors to cytosolic MAP kinase cascades. Members of this subfamily include KHS, GLK, YSK1, HPK1, Krs-1, Krs-2, GC kinase, HGK and SLK. SLK is a ubiquitously expressed protein that localizes to the cytoplasm and contains an N-terminal protein kinase domain, a central coiled-coil domain and a C-terminal ATH domain. SLK is activated through cleavage by caspase-3. SLK indirectly associates with microtubules and plays an important role in cellular stress, cell motility, cell death and cytoskeletal dynamics.

Additional Information
Gene ID	9748
Other Names	LOSK; STK2; bA16H23.1; se20-9; STE20 like kinase; SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase (yeast); STE20-like kinase (yeast); STE20-like kinase
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Name	SLK Rabbit pAb
Function	SLK is a serine/threonine protein kinase that is involved in cytoskeletal reorganization and apoptosis. SLK is a microtubule-associated protein inducing actin stress fiber disassembly, it also mediates apoptosis and it is ubiquitously expressed; highest expression is found in heart and in skeletal muscle. There are two named isoforms.