癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
CD80 Mouse mAb
- 产品详情
- 实验流程
Application 
| WB |
|---|---|
| Primary Accession | Q00609 |
| Other Accession | Q00609 |
| Reactivity | Human, Mouse |
| Host | Mouse |
| Clonality | Monoclonal |
| Calculated MW | 34590 Da |
| Physical State | Liquid |
| Immunogen | Recombinant mouse CD80 protein |
| Epitope Specificity | 38-246/306 |
| Isotype | IgG1 |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines. |
| Gene ID | 12519 |
|---|---|
| Other Names | B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; B71; Cd28l; Ly-53; Ly53; MIC17; TSA1; CD80_HUMAN; CD80; Activation B7-1 antigen; CTLA-4 counter-receptor B7.1 (B7); CD80_MOUSE; Activation B7-1 antigen (B7); CD80 molecule; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B-lymphocyte activation antigen B7 |
| Dilution | ICC/IF=1:100-500,Flow-Cyt=1ug/Test,WB=1:500-2000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Cd80 |
|---|---|
| Synonyms | B7 |
| Function | Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed:16339518, PubMed:1714935). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:16339518). In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (By similarity). Also acts as an inhibitor of T- cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming (By similarity). In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (By similarity). Also acts as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (By similarity). Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (PubMed:22450810). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P33681} |
| Tissue Location | Expressed on activated B-cells, gamma interferon stimulated monocytes and non-circulating B-cell malignancies |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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