癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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phospho-HMG14 (Ser20+Ser24) Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | P05114 |
| Other Accession | P05114 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 10659 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human HMG14 around the phosphorylation site of Ser20+Ser24 |
| Epitope Specificity | RR(p-S)ARL(p-S)AK |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the HMGN family. |
| Post-translational modifications | Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011] |
| Gene ID | 3150 |
|---|---|
| Other Names | HMG14; HMG-14; HMGN1_BOVIN; HMGN1; High mobility group nucleosome-binding domain-containing protein 1; HMGN1_HUMAN; HMGN1_MOUSE; high mobility group nucleosome binding domain 1; high-mobility group (nonhistone chromosomal) protein 14; high-mobility group nucleosome binding domain 1; high-mobility group nucleosome binding 1; nonhistone chromosomal protein HMG-14; HMGN1 | HMG14 (phospho-S20/S24); p-HMG14; phospho-HMG14; p-HMGN1; HMGN1 | HMG14 (phospho-Ser20/Ser24) |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | HMGN1 |
|---|---|
| Synonyms | HMG14 |
| Function | Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 (By similarity). |
| Cellular Location | Nucleus. Cytoplasm. Note=Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP96983
