phospho-HSL (Ser660) Rabbit pAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Primary Accession	P54310
Other Accession	P54310
Host	Rabbit
Clonality	Polyclonal
Calculated MW	83348 Da
Physical State	Liquid
Immunogen	KLH conjugated synthesised phosphopeptide derived from mouse HSL around the phosphorylation site of Ser660
Epitope Specificity	SK(p-S)HE
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY	Belongs to the 'GDXG' lipolytic enzyme family.
SUBUNIT	Interacts with PTRF in the adipocyte cytoplasm.
Post-translational modifications	Phosphorylation by AMPK may block translocation to lipid droplets.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	HSL/LIPE is found in adipose tissue and heart, where it primarily hydrolyzes stored triglycerides to free fatty acids. It is also found in steroidogenic tissues, where it principally converts cholesteryl esters to free cholesterol for steroid hormone production. There are two named isoforms.

Additional Information
Gene ID	16890
Other Names	AOMS4; FPLD6; HSL; LHS; REH; 4933403G17Rik; LIPS_HUMAN; LIPE; Monoacylglycerol lipase LIPE; Retinyl ester hydrolase (REH); 3.1.1.79; Q6NSL7_HUMAN; LIPS_MOUSE; LIPS_RAT; lipase E, hormone sensitive type; lipase, hormone-sensitive; LIPE \| HSL (phospho-S660); p-HSL; phospho-HSL; p-LIPE; LIPE \| HSL (phospho-Ser660)
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	Lipe
Function	Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed:15550674, PubMed:20625037, PubMed:21454566, PubMed:23066022, PubMed:23291629). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and of the fatty acid (FA) esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Preferentially hydrolyzes FA esters at the sn-3 position of the glycerol backbone in DAGs (PubMed:23066022). Catalyzes the hydrolysis of 2- arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (PubMed:20625037, PubMed:21454566). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity).
Cellular Location	Cell membrane. Membrane, caveola {ECO:0000250\|UniProtKB:Q05469}. Cytoplasm, cytosol. Lipid droplet. Note=Found in the high-density caveolae (By similarity). Translocates to the cytoplasm from the caveolae upon insulin stimulation (By similarity). Phosphorylation by AMPK reduces its translocation towards the lipid droplets {ECO:0000250\|UniProtKB:Q05469, ECO:0000269\|PubMed:15878856}