癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PPM1D Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | O15297 |
| Other Accession | O15297 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 66675 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human PPM1D |
| Epitope Specificity | 471-570/605 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the PP2C family. Contains 1 PP2C-like domain. |
| SUBUNIT | Interacts with CHEK1 and CHEK2; dephosphorylates them. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | PPM1D (Wip) is a serine/threonine phosphatase implicated in cell cycle control, spermatogenesis, and lymphoid cell function. The predicted 605-amino acid PPM1D protein contains 2 putative nuclear localization signals and 3 regions conserved in serine/threonine PP2C phosphatases, as well as characteristics of a type 2C phosphatase, including magnesium dependence and relative insensitivity to okadaic acid. PPM1D expression is induced in response to ionizing radiation in a p53-dependent manner. The accumulation of PPM1D mRNA following ionizing radiation is rapid and transient, and PPM1D protein is localized to the nucleus. PPM1D may contribute to growth inhibitory pathways activated in response to DNA damage in a p53-dependent manner. PPM1D inhibits phosphorylation of the p38 mitogen-activated (MAP)kinase protein. Through p38 MAPK, PPM1D modulates the CDKN2A tumor-suppressor locus. This gene is located in a chromosomal region known to be amplified in breast cancer, (located at 17q22-q23), is amplified in human breast tumor cell lines and in approximately 11% of primary breast tumors, and appears to lead to cell transformation by abrogating p53 tumor suppressor activity. Inactivation of the p38 MAPK through PPM1D overexpression resulting from PPM1D amplification may contributes to the development of human cancers by suppressing p53 activation. PPM1D null mice have increased susceptibility to pathogens and reduced male fertility and longevity. |
| Gene ID | 8493 |
|---|---|
| Other Names | IDDGIP; JDVS; PP2C-DELTA; WIP1; XELAEV_180152921mg; ppm1d; wu:fj44c08; zgc:55836; zgc:77316; PPM1D_HUMAN; Protein phosphatase 2C isoform delta (PP2C-delta); Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1; 3.1.3.16; Q5RI25_MOUSE; PPM1D_MOUSE; protein phosphatase, Mg2+/Mn2+ dependent 1D; protein phosphatase 1D magnesium-dependent, delta isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1D; wild-type p53-induced phosphatase 1; protein phosphatase 2C, delta isoform |
| Dilution | IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PPM1D |
|---|---|
| Synonyms | WIP1 |
| Function | Involved in the negative regulation of p53 expression (PubMed:23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed:15870257, PubMed:16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed:21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity). |
| Cellular Location | Nucleus. Cytoplasm, cytosol |
| Tissue Location | Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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