癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Orexin Prepro Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | O43612 |
| Other Accession | O43612 |
| Predicted | Rat, Pig, Human, Mouse, Rabbit, Dog, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 13363 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human Orexin Prepro |
| Epitope Specificity | 70-97/131 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the orexin family. |
| Post-translational modifications | Specific enzymatic cleavages at paired basic residues yield the different active peptides. |
| DISEASE | Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1) [MIM:161400]. Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Note=Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis. These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R. Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus. Orexin A and Orexin B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. |
| Gene ID | 3060 |
|---|---|
| Other Names | NRCLP1; OX; PPOX; OREX_HUMAN; HCRT; Hypocretin (Hcrt); Orexin precursor; Prepro-orexin; Preprohypocretin; PPORX; hypocretin neuropeptide precursor; hypocretin (orexin) neuropeptide precursor; orexin |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | HCRT (HGNC:4847) |
|---|---|
| Synonyms | OX, PPORX, PPOX |
| Function | Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. |
| Cellular Location | Rough endoplasmic reticulum {ECO:0000250|UniProtKB:O55232}. Cytoplasmic vesicle {ECO:0000250|UniProtKB:O55232}. Synapse {ECO:0000250|UniProtKB:O55232} Note=Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses {ECO:0000250|UniProtKB:O55232} |
| Tissue Location | Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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