癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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eNOS Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | P70313 |
| Other Accession | P70313 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 132916 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from mouse eNOS |
| Epitope Specificity | 1105-1202/1203 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. |
| SUBUNIT | Homodimer. Interacts with NOSIP and NOSTRIN. |
| Post-translational modifications | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). |
| Gene ID | 18127 |
|---|---|
| Other Names | ECNOS; eNOS; 2310065A03Rik; Nos-3; NOS3_BOVIN; NOS3; Constitutive NOS (cNOS); EC-NOS; NOS type III (NOSIII); Nitric oxide synthase, endothelial (Endothelial NOS | eNOS); 1.14.13.39; NOS3_HUMAN; NOS3_MOUSE; NOS3_RAT; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); endothelial nitric oxide synthase |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Nos3 {ECO:0000312|MGI:MGI:97362} |
|---|---|
| Synonyms | Ecnos |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development. |
| Cellular Location | Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Cell membrane. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP98323
