癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Gli2 Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | P10070 |
| Other Accession | P10070 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 167783 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human Gli2 |
| Epitope Specificity | 501-600/1586 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the GLI C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers. |
| SUBUNIT | Interaction with ZIC |
| Post-translational modifications | Phosphorylated in vitro by ULK3. |
| DISEASE | Defects in GLI2 are the cause of holoprosencephaly type 9 (HPE9) [MIM:610829]; also called pituitary anomalies with holoprosencephaly-like features. The primary features of this disease include defective anterior pituitary formation and pan-hypopituitarism, with or without overt forebrain cleavage abnormalities, and holoprosencephaly-like midfacial hypoplasia. Holoprosencephaly is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | It has long been known that the overexpression of either Wnt-1 or the GLI proteins results in cancer; however, the molecular basis for this transformation was poorly understood. The Wnt-1 and GLI proteins have now been placed in a signaling cascade downstream of the mammalian homologs of the Drosophila hedgehog and patched proteins. The Drosophila segment polarity gene hedgehog (hh) encodes a secreted protein that appears to function in embryonic and imaginal disc patterning. The ptc gene, also identified as a Drosophila segment polarity gene, encodes the transmembrane protein patched, the expression of which is precisely regulated during embryonic development. Hedgehog has been shown to enhance the expression of the Wnt family of proteins through a signaling cascade involving the GLI transcription factors, while patched functions as a repressor opposing the effects of hedgehog. Mutations in the ptc gene, which result in unregulated hedgehog signaling, have been correlated with the most common type of cancer, basal cell carcinoma, which affects 750,000 individuals annually in the United States alone. |
| Gene ID | 2736 |
|---|---|
| Other Names | CJS; HPE9; PHS2; THP1; THP2; GLI2_HUMAN; GLI2; GLI family zinc finger protein 2; Tax helper protein; THP; GLI2_MOUSE; Q8K0K3_MOUSE; GLI family zinc finger 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; tax-responsive element-2 holding protein; tax helper protein 1; tax helper protein 2 |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | GLI2 {ECO:0000303|PubMed:9557682, ECO:0000312|HGNC:HGNC:4318} |
|---|---|
| Function | Transcription factor that acts as a key effector of the smoothened signaling pathway, and which plays a role in skeleton development (PubMed:15994174, PubMed:18455992, PubMed:19878745, PubMed:20154143, PubMed:20685856, PubMed:24311597, PubMed:26565916, PubMed:9557682). Specifically binds to the minimal GLI-consensus sequence 5'-GACCACCCA-3' to activate or repress the expression of target genes (By similarity). Functions both as a transcriptional activator and repressor: the full-length GLI2 form (Transcription activator GLI2) acts as a transcription activator following smoothened activation, while the Transcription repressor GLI2R, which is generated in absence of smoothened, acts as a transcription repressor (By similarity). The transcriptional activator form constitutes the major form of GLI2 (By similarity). |
| Cellular Location | Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q0VGT2} Cell projection, cilium. Note=Full-length GLI2 is sequestered in the cytoplasm by SUFU in absence of smoothened signaling. {ECO:0000250|UniProtKB:Q0VGT2} [Transcription repressor GLI2R]: Nucleus {ECO:0000250|UniProtKB:Q0VGT2}. Note=Following generation by proteolytic processing in absence of smoothened, translocates to the nucleus, where is represses transcription of target genes {ECO:0000250|UniProtKB:Q0VGT2} [Isoform 2]: Nucleus |
| Tissue Location | Expressed in breast cancers (at protein level) (PubMed:26565916). Isoform 1 and isoform 4 are expressed in HTLV-1- infected T-cell lines (at protein level) (PubMed:9557682). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines (PubMed:9557682). Isoform 3 and isoform 4 are weakly expressed in HTLV- 1-infected T-cell lines (PubMed:9557682). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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