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MARCH5 Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q9NX47 |
| Other Accession | Q9NX47 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 31232 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human MARCH5 |
| Epitope Specificity | 1-100/278 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Contains 1 RING-CH-type zinc finger. |
| SUBUNIT | Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1. |
| Post-translational modifications | Autoubiquitinated leading to degradation (short half-life). |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | MARCH5 is a member of the E3 ubiquitin-protein ligase family. This mitochonrial E3 ubiquitin-protein ligase plays an importnat role in controlling the morphology of the mitochondria. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH5 has been shown to interact with MTFN2 and ubiquitinated forms of DRP1. |
| Gene ID | 54708 |
|---|---|
| Other Names | MARCH-V; MARCH5; MITOL; RNF153; 1810015H18Rik; 2310008I22Rik; 2700055A20Rik; 5730499H23Rik; E130202O05Rik; Marchv; MARH5_HUMAN; MARCHF5; Membrane-associated RING finger protein 5; Membrane-associated RING-CH protein V (MARCH-V); Mitochondrial ubiquitin ligase (MITOL); RING finger protein 153; RING-type E3 ubiquitin transferase MARCHF5; 2.3.2.27; membrane associated ring-CH-type finger 5; membrane-associated ring finger (C3HC4) 5; mitochondrial ubiquitin ligase; E3 ubiquitin-protein ligase MARCHF5 |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | MARCHF5 (HGNC:26025) |
|---|---|
| Synonyms | MARCH5, RNF153 |
| Function | Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission and as an important regulator of immune response (PubMed:16874301, PubMed:17606867, PubMed:26246171, PubMed:31881323). Plays a crucial role in maintaining mitochondrial homeostasis by regulating the dynamics of mitochondria through the ubiquitination of key proteins involved in fission and fusion such as FIS1, DNM1L and MFN1 (PubMed:16874301, PubMed:17606867). Acts as a critical determinant of mitotic apoptosis through both MCL1- dependent and -independent pathways (By similarity). Turns off persistent immune signaling by degrading oligomeric complexes of retinoic acid-inducible gene I/DDX58 and mitochondrial antiviral- signaling protein/MAVS formed upon RNA virus infection (PubMed:26246171, PubMed:31881323, PubMed:40071916). Promotes STING- mediated type-I interferon production via 'Lys-63'-linked ubiquitination of STING1 thereby preserving its activity and preventing the formation of inactive STING1 polymers (PubMed:37916870). Plays also an essential role in the formation of PEX3-containing vesicles in the de novo biogenesis of peroxisomes from mitochondria (PubMed:39423820, PubMed:39423819). Acts as a regulator of NLRP3 inflammasome activation on the mitochondria by mediating the 'Lys-27'-linked polyubiquitination of NLRP3, positively regulating the NLRP3-NEK7 complex formation and NLRP3 oligomerization (PubMed:37575012). |
| Cellular Location | Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Peroxisome membrane. Note=Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301). |
| Tissue Location | Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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