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ACBG2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ACBG2 Antibody (Center) AP9854c
    Anti-ACBG2 Antibody (Center) at 1:1000 dilution + human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 74 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ACBG2 Antibody (Center) AP9854c
    Western blot analysis of ACBG2 Antibody (Center) (Cat. #AP9854c) in K562 cell line lysates (35ug/lane). ACBG2 (arrow) was detected using the purified Pab.
  • 14 - ACBG2 Antibody (Center) AP9854c
    ACBG2 Antibody (Center) (Cat. #AP9854c) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACBG2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - ACBG2 Antibody (Center) AP9854c
    ACBG2 Antibody (Center) (Cat. #AP9854c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q5FVE4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 74354 Da
Antigen Region 237-265 aa
Additional Information
Gene ID 81616
Other Names Long-chain-fatty-acid--CoA ligase ACSBG2, Acyl-CoA synthetase bubblegum family member 2, Bubblegum-related protein, PRTD-NY3, ACSBG2, BGR
Target/Specificity This ACBG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-265 amino acids from the Central region of human ACBG2.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACBG2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACSBG2 (HGNC:24174)
Function Catalyzes the conversion of fatty acids such as long chain and very long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids (PubMed:16371355, PubMed:16762313). Has increased ability to activate oleic and linoleic acid (PubMed:16371355). May play a role in spermatogenesis (PubMed:15685348).
Cellular Location Cytoplasm. Membrane; Peripheral membrane protein.
Tissue Location Testis-specific.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

Fraisl, P., et al. Arch. Biochem. Biophys. 451(1):23-33(2006)
Pei, Z., et al. J. Biol. Chem. 281(10):6632-6641(2006)
Zheng, Y., et al. Asian J. Androl. 7(1):21-32(2005)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)

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