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ZNF274 Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | Q96GC6 |
| Other Accession | Q96GC6 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 74177 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human ZNF274 |
| Epitope Specificity | 251-350/653 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers. Contains 2 KRAB domains. Contains 1 SCAN box domain. |
| SUBUNIT | Interacts with SETDB1 and TRIM28/KAP1. |
| Post-translational modifications | Ubiquitinated by TRAF6 at Lys-15 through 'Lys-63'-linked polyubiquitination. 'Lys-63'-linked polyubiquitination occurs in response to NGFR/p75(NTR) cleavage by gamma-secretase and promotes binding with the ICD cleavage product of NGFR/p75(NTR), followed by tranlocation into the nucleus and subsequent apoptosis. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008] 74kDa(hu); 94kDa(mo,rat); |
| Gene ID | 10782 |
|---|---|
| Other Names | HFB101; ZF2; ZKSCAN19; ZSCAN51; ZN274_HUMAN; ZNF274; Zinc finger protein 274; Zinc finger protein HFB101; Zinc finger protein with KRAB and SCAN domains 19; Zinc finger protein zfp2 (Zf2) |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ZNF274 |
|---|---|
| Synonyms | ZKSCAN19 |
| Function | Probable transcription repressor. Specifically binds to the 3'-end of zinc-finger coding genes and recruiting chromatin-modifying proteins such as SETDB1 and TRIM28/KAP1, leading to transcription repression. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). |
| Cellular Location | Cytoplasm. Nucleus, nucleolus |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP98619
