ACOT13 Rabbit pAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q9NPJ3
Other Accession	Q9NPJ3
Host	Rabbit
Clonality	Polyclonal
Calculated MW	14960 Da
Physical State	Liquid
Immunogen	Recombinant human ACOT13 protein
Epitope Specificity	1-140/140
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY	Belongs to the thioesterase PaaI family.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009]

Additional Information
Gene ID	55856
Other Names	CK820_G0045331; THEM2; HT012; PNAS-27; 0610006O17Rik; CR201_G0046122; ACO13_HUMAN; ACOT13; Acyl-CoA thioesterase 13; Hotdog-fold thioesterase superfamily member 2; Palmitoyl-CoA hydrolase; Thioesterase superfamily member 2 (THEM2); 3.1.2.-; ACO13_MOUSE; ACO13_PONAB; thioesterase superfamily member 2
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ACOT13 (HGNC:20999)
Synonyms	THEM2
Function	Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed:16934754, PubMed:19170545). Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates (By similarity) (PubMed:16934754, PubMed:19170545). Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro) (By similarity) (PubMed:16934754, PubMed:19170545). May play a role in controlling adaptive thermogenesis (By similarity).
Cellular Location	Cytoplasm, cytosol {ECO:0000250\|UniProtKB:Q9CQR4}. Mitochondrion {ECO:0000250\|UniProtKB:Q9CQR4}. Nucleus {ECO:0000250\|UniProtKB:Q9CQR4} Cytoplasm, cytoskeleton, spindle {ECO:0000250\|UniProtKB:Q9CQR4} Note=During interphase, found both in the nucleus and in the cytoplasm At mitosis, localizes to the spindle. Colocalizes with tubulin {ECO:0000250\|UniProtKB:Q9CQR4}