癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PHF2 Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | O75151 |
| Other Accession | O75151 |
| Reactivity | Rat, Human, Mouse |
| Predicted | Pig, Rabbit, Dog, Horse, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 120775 Da |
| Physical State | Liquid |
| Immunogen | Recombinant human PHF2 protein |
| Epitope Specificity | 551-762/1096 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily. Contains 1 JmjC domain. Contains 1 PHD-type zinc finger. |
| SUBUNIT | Component of the PHF2-ARID5B complex, at least composedof PHF2 and ARID5B. Interacts with HNF4A and NR1H4. |
| Post-translational modifications | Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | This gene encodes a protein which contains a zinc finger-like PHD (plant homeodomain) finger, distinct from other classes of zinc finger motifs, and a hydrophobic and highly conserved domain. The PHD finger shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. [provided by RefSeq, Jul 2008] |
| Gene ID | 5253 |
|---|---|
| Other Names | CENP-35; GRC5; JHDM1E; KDM7C; PHF2_HUMAN; PHF2; PHD finger protein 2; 1.14.11.-; KIAA0662; PHF2_MOUSE; lysine demethylase 7C; jumonji C domain-containing histone demethylase 1E; centromere protein 35 |
| Dilution | IHC-P=1:100-500,WB=1:500-2000,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PHF2 (HGNC:8920) |
|---|---|
| Function | Lysine demethylase that demethylates both histones and non- histone proteins (PubMed:20129925, PubMed:21167174, PubMed:21532585). Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B (PubMed:21532585). Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes (PubMed:21532585). The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA (PubMed:21532585). Involved in the activation of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the demethylation of trimethylated histone H4 lysine 20 (H4K20me3) at the gene promoters (By similarity). |
| Cellular Location | Nucleus, nucleolus. Chromosome, centromere, kinetochore |
| Tissue Location | Widely expressed, including in liver (at protein level). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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