癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
IRS-4 Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q9Z0Y7 |
| Other Accession | Q9Z0Y7 |
| Reactivity | Rat |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 130147 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from mouse IRS-4 |
| Epitope Specificity | 401-500/1216 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Contains 1 IRS-type PTB domain. Contains 1 PH domain. |
| SUBUNIT | Interacts with CRK and CRKL. Interaction with CRK is stronger than with CRKL. Interacts with CRK via the phosphorylated YXXM motifs. Interacts with PLC-gamma, SHC1, PTK6, PPP4C and NISCH. Interacts with SOCS6 in response to stimulatiom with either insulin or IGF1. Interacts with PIK3R1 and GRB2. |
| Post-translational modifications | Phosphorylated on tyrosine residues in response to both insulin and IGF1 signaling. Phosphorylated on Tyr-894 in response to FGF2 signaling. Phosphorylation of Tyr-894 is required for GRB2, phospholipase C-gamma and phosphatidylinositol 3-kinase interaction. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | IRS4 (Insulin receptor substrate 4) is a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signaling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation. |
| Gene ID | 16370 |
|---|---|
| Other Names | CHNG9; IRS-4; PY160; IRS4_HUMAN; IRS4; 160 kDa phosphotyrosine protein (py160); Phosphoprotein of 160 kDa (pp160); IRS4_MOUSE; insulin receptor substrate 4 |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Irs4 |
|---|---|
| Function | Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin- stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2. |
| Cellular Location | Cell membrane; Peripheral membrane protein; Cytoplasmic side |
| Tissue Location | Expressed in skeletal muscle, brain, heart, kidney and liver. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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