癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DFFB Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | O54788 |
| Other Accession | O54788 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 39449 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from mouse DFF-40 beta |
| Epitope Specificity | 201-260/344 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Contains 1 CIDE-N domain. |
| SUBUNIT | Heterodimer of DFFA and DFFB. Interacts with HIST1H1A. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq, Jul 2008]. |
| Gene ID | 13368 |
|---|---|
| Other Names | CAD; CPAN; DFF-40; DFF2; DFF40; 40kDa; 5730477D02Rik; Didff; DFFB_HUMAN; DFFB; Caspase-activated deoxyribonuclease (CAD | Caspase-activated DNase); Caspase-activated nuclease (CPAN); DNA fragmentation factor 40 kDa subunit (DFF-40); 3.-.-.-; DFFB_MOUSE; DFFB_RAT; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); caspase-activated DNase |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Dffb |
|---|---|
| Synonyms | Cad |
| Function | Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. |
| Cellular Location | Cytoplasm. Nucleus. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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