- 文献引用 : 2
|Application ||WB, FC, E|
|Other Accession||Q7TMY4, Q3SZ60|
|Calculated MW||23743 Da|
|Other Names||THO complex subunit 7 homolog, Functional spliceosome-associated protein 24, fSAP24, Ngg1-interacting factor 3-like protein 1-binding protein 1, NIF3L1-binding protein 1, hTREX30, THOC7, NIF3L1BP1|
|Target/Specificity||This THOC7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-166 amino acids from the C-terminal region of human THOC7.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||THOC7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway.|
|Cellular Location||Cytoplasm. Nucleus. Nucleus speckle Note=Interaction with THOC5 is required for nuclear localization|
Author : Ramachandran S1, Tran DD, Klebba-Faerber S, Kardinal C, Whetton AD, Tamura T.
RNA. 2011 Nov;17(11):1957-66. doi: 10.1261/rna.2820911. Epub 2011 Sep 21.
Author : Lee JG, Song JS, Smith RE, Kay EP.
Invest Ophthalmol Vis Sci. 2011 Oct 17;52(11):8216-23. doi: 10.1167/iovs.11-8213.
Provided below are standard protocols that you may find useful for product applications.
El Bounkari, O., et al. FEBS Lett. 583(1):13-18(2009)
Boyne, J.R., et al. PLoS Pathog. 4 (10), E1000194 (2008)
Cheng, H., et al. Cell 127(7):1389-1400(2006)
Masuda, S., et al. Genes Dev. 19(13):1512-1517(2005)