癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
phospho-AQP2 (Ser269) Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | P56402 |
| Other Accession | P56402 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 28965 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from mouse AQP2 around the phosphorylation site of Ser269 |
| Epitope Specificity | RG(p-S)KA |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the MIP/aquaporin (TC 1.A.8) family. |
| Post-translational modifications | Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. |
| DISEASE | Defects in AQP2 are the cause of diabetes insipidus nephrogenic autosomal (ANDI) [MIM:125800]; also known as diabetes insipidus nephrogenic type 2. ANDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant, and recessive forms of nephrogenic diabetes insipidus. Belongs to the MIP/aquaporin (TC 1.A.8) family. |
| Gene ID | 11827 |
|---|---|
| Other Names | AQP-CD; NDI2; WCH-CD; cph; jpk; AQP-2; aquaporin-2; AQP2_HUMAN; AQP2; ADH water channel; Aquaporin-CD (AQP-CD); Collecting duct water channel protein; Water channel protein for renal collecting duct; AQP2_MOUSE; AQP2_RAT; aquaporin 2; aquaporin 2 (collecting duct); AQP2 (phospho-S269); p-AQP2; phospho-AQP2; AQP2 (phospho-Ser269) |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Aqp2 {ECO:0000312|MGI:MGI:1096865} |
|---|---|
| Function | Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (PubMed:10191086). Plays an essential role in renal water homeostasis (PubMed:16641094). Could also be permeable to glycerol (By similarity). |
| Cellular Location | Apical cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P41181}. Basolateral cell membrane {ECO:0000250|UniProtKB:P34080}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P41181}. Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P41181}. Cytoplasmic vesicle membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P41181}. Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:P41181}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P41181}. Note=Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane (PubMed:16735444) PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated. {ECO:0000250|UniProtKB:P41181, ECO:0000269|PubMed:16735444} |
| Tissue Location | Detected in principal cells in the collecting duct in kidney medulla and cortex (at protein level) (PubMed:10191086, PubMed:16641094, PubMed:16735444, PubMed:31605441). Expressed in a radial pattern from the cortex through the outer medulla into the inner medulla (PubMed:12426236). Higher levels in the inner medulla (PubMed:12426236). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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