TRIM56 Recombinant Rabbit mAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Primary Accession	Q9BRZ2
Other Accession	Q9BRZ2
Host	Rabbit
Clonality	Recombinant
Calculated MW	81488 Da
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY	Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 RING-type zinc finger.
SUBUNIT	Interacts with TMEM173/STING (By similarity).
Background Descriptions	sequence caution:Translation N-terminally extended.,sequence caution:Translation N-terminally shortened.,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 2 B box-type zinc fingers.,

Additional Information
Gene ID	81844
Other Names	RNF109; TRI56_HUMAN; TRIM56; RING finger protein 109; Tripartite motif-containing protein 56; 2.3.2.27; tripartite motif containing 56; tripartite motif-containing 56; E3 ubiquitin-protein ligase TRIM56 (RING finger protein 109) (Tripartite motif-containing protein 56)
Dilution	IHC-P=1:50-200,IHC-F=1:50-200,WB=1:500-2000,IF=1:50-200,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TRIM56 {ECO:0000303\|PubMed:21289118, ECO:0000312\|HGNC:HGNC:19028}
Function	E3 ubiquitin-protein ligase that plays a key role in innate antiviral immunity by mediating ubiquitination of CGAS and STING1 (PubMed:21289118, PubMed:29426904). In response to pathogen- and host- derived double-stranded DNA (dsDNA), targets STING1 to 'Lys-63'-linked ubiquitination, thereby promoting its homodimerization, a step required for the production of type I interferon IFN-beta (By similarity). Also mediate monoubiquitination of CGAS, thereby promoting CGAS oligomerization and subsequent activation (PubMed:29426904). Promotes also TNFalpha-induced NF-kappa-B signaling by mediating 'Lys-63'-linked ubiquitination TAK1, leading to enhanced interaction between TAK1 and CHUK/IKKalpha (PubMed:35952808). Independently of its E3 ubiquitin ligase activity, positive regulator of TLR3 signaling. Potentiates extracellular double stranded RNA (dsRNA)-induced expression of IFNB1 and interferon-stimulated genes ISG15, IFIT1/ISG56, CXCL10, OASL and CCL5/RANTES (PubMed:22948160). Promotes establishment of an antiviral state by TLR3 ligand and TLR3-mediated chemokine induction following infection by hepatitis C virus (PubMed:22948160). Acts as a restriction factor of Zika virus through direct interaction with the viral RNA via its C-terminal region (PubMed:31251739).
Cellular Location	Cytoplasm.
Tissue Location	Widely expressed (at protein level).