癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
HSL/LIPE Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | P15304 |
| Other Accession | P15304 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 116812 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from rat HSL |
| Epitope Specificity | 261-360/1068 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the 'GDXG' lipolytic enzyme family. |
| SUBUNIT | Interacts with PTRF in the adipocyte cytoplasm. |
| Post-translational modifications | Phosphorylation by AMPK may block translocation to lipid droplets. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | HSL/LIPE is found in adipose tissue and heart, where it primarily hydrolyzes stored triglycerides to free fatty acids. It is also found in steroidogenic tissues, where it principally converts cholesteryl esters to free cholesterol for steroid hormone production. There are two named isoforms. |
| Gene ID | 25330 |
|---|---|
| Other Names | AOMS4; FPLD6; HSL; LHS; REH; 4933403G17Rik; LIPS_HUMAN; LIPE; Monoacylglycerol lipase LIPE; Retinyl ester hydrolase (REH); 3.1.1.79; Q6NSL7_HUMAN; LIPS_MOUSE; LIPS_RAT; lipase E, hormone sensitive type; lipase, hormone-sensitive |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Lipe |
|---|---|
| Function | Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed:10694408, PubMed:6643478, PubMed:9162045). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of DAGs (By similarity). Preferentially hydrolyzes fatty acids at the sn-1 and sn-2 positions of TAGs (PubMed:6643478). Catalyzes the hydrolysis of 2- arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (PubMed:1770312). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:Q05469}. Membrane, caveola {ECO:0000250|UniProtKB:Q05469}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q05469}. Lipid droplet. Note=Found in the high-density caveolae (By similarity). Translocates to the cytoplasm from the caveolae upon insulin stimulation (By similarity). Phosphorylation by AMPK reduces its translocation towards the lipid droplets {ECO:0000250|UniProtKB:Q05469, ECO:0000269|PubMed:15878856} |
| Tissue Location | Highly expressed in the adipose tissue (PubMed:1770312). Also expressed in the heart, adrenal gland and testis (PubMed:1770312, PubMed:8812477). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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