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DBF4A Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | Q9UBU7 |
| Other Accession | Q9UBU7 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 76858 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human DBF4A |
| Epitope Specificity | 21-110/674 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Contains 2 BRCT domains. Contains 1 DBF4-type zinc finger. |
| SUBUNIT | Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6. |
| Post-translational modifications | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. Tissue specificity: Highly expressed in testis and thymus. Expressed also in most cancer cells lines. |
| Gene ID | 10926 |
|---|---|
| Other Names | DBF4A_HUMAN; DBF4; Activator of S phase kinase; Chiffon homolog A; DBF4-type zinc finger-containing protein 1; ASK; DBF4A; ZDBF1; DBF4A_MOUSE; MuDBF4; DBF4_YEAST; Dumbbell forming protein 4; DNA52; CHIF; RGD1305854; DBF4-CDC7 kinase regulatory subunit; DBF4 homolog (S. cerevisiae); DBF4 zinc finger; chiffon homolog (Drosophila); zinc finger, DBF-type containing 1; DBF4 zinc finger A; regulatory subunit of DDK |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | DBF4 |
|---|---|
| Synonyms | ASK, DBF4A, ZDBF1 |
| Function | Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7- DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle. |
| Cellular Location | Nucleus. |
| Tissue Location | Highly expressed in testis and thymus. Expressed also in most cancer cells lines. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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¥ 1,250.00
Cat# AP99408
