癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SPRED2 Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q7Z698 |
| Other Accession | Q7Z698 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 47558 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human SPRED2 |
| Epitope Specificity | 381-480/674 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Contains 1 KBD domain.Contains 1 SPR (sprouty) domain. Contains 1 WH1 domain. |
| SUBUNIT | Interacts with Ras (By similarity). Homodimer and heterodimer. Able to interact with SPRED1 to form heterodimers. |
| Post-translational modifications | Phosphorylated on tyrosine. Phosphorylation of Tyr-228 and Tyr-231 are required for ubiquitination.Ubiquitinated; leading to degradation by the proteasome. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM, Mar 2008] |
| Gene ID | 200734 |
|---|---|
| Other Names | NS14; Spred-2; SPRE2_HUMAN; SPRED2; SPRE2_MOUSE; SPRE2_RAT; sprouty related EVH1 domain containing 2 |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | SPRED2 |
|---|---|
| Function | Negatively regulates Ras signaling pathways and downstream activation of MAP kinases (PubMed:15683364, PubMed:34626534). Recruits and translocates NF1 to the cell membrane, thereby enabling NF1- dependent hydrolysis of active GTP-bound Ras to inactive GDP-bound Ras (PubMed:34626534). Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (By similarity). Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (By similarity). |
| Cellular Location | Cell membrane; Peripheral membrane protein {ECO:0000250|UniProtKB:Q924S7}; Cytoplasmic side {ECO:0000250|UniProtKB:Q924S7}. Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Note=Detected in the cytoplasm of the stratum spinosum cells, where it is associated with cytoplasmic vesicles that are supposed to be secretory granules |
| Tissue Location | Expressed in liver, skin, small intestine, salivary gland and prostate. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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