癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
RECQL5 Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | O94762 |
| Other Accession | O94762 |
| Predicted | Rat, Pig, Human, Mouse, Dog, Horse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 108858 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human RECQL5 |
| Epitope Specificity | 1-100/991 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the helicase family. RecQ subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. |
| SUBUNIT | Interacts with TOP3A and TOP3B. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | RecQ helicases are implicated in DNA recombination and repair helicases and are critical in genome maintenance. RecQL5 is one of five known RecQ homologues in humans, and defects in three of these (BLM, WRN and RecQ4)are associated with autosomal recessive disorders that involve genomic instability and a predisposition to cancer. The exact role of ReqL5 is unclear. It has been shown in mouse embryonic stem (ES) cells that mutations in the Recql5 genes result in a significant increase in the frequency of sister chromatid exchange (SCE), whereas deleting Recql5 lead to an even higher frequency of SCE. There are three different RecQL5 isoforms - alpha, beta and gamma, produced by alternative splicing. |
| Gene ID | 9400 |
|---|---|
| Other Names | RECQ5; RECQ5_HUMAN; RECQL5; DNA 3'-5' helicase RecQ5; DNA helicase, RecQ-like type 5 (RecQ5); RecQ protein-like 5; 5.6.2.4; RecQ like helicase 5; RecQ protein 5 |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | RECQL5 |
|---|---|
| Synonyms | RECQ5 {ECO:0000303|PubMed:9878247} |
| Function | DNA helicase that plays an important role in DNA replication, transcription and repair (PubMed:20643585, PubMed:22973052, PubMed:28100692). Probably unwinds DNA in a 3'-5' direction (Probable) (PubMed:28100692). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability (PubMed:20231364). Also associates with POLR1A and enforces the stability of ribosomal DNA arrays (PubMed:27502483). Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress (PubMed:22013166). Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81-EME1 endonuclease leading to mitotic DNA synthesis (PubMed:28575661). Required for efficient DNA repair, including repair of inter-strand cross-links (PubMed:23715498). Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination. A core helicase fragment (residues 11-609) binds preferentially to splayed duplex, looped and ssDNA (PubMed:28100692). |
| Cellular Location | [Isoform Beta]: Nucleus, nucleoplasm. Nucleus Note=Recruited to sites of DNA damage, such as single-strand breaks and inter-strand cross-links, and at stalled replication forks. Re- localizes from the nucleolus into the nucleus after replicative stress and significantly associates with WRN during S-phase (PubMed:23180761) [Isoform Gamma]: Cytoplasm |
| Tissue Location | Ubiquitous.. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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