癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
AADAC Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | P22760 |
| Other Accession | P22760 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 45734 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human AADAC |
| Epitope Specificity | 228-273/399 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the 'GDXG' lipolytic enzyme family. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | The assembly of very-low-density lipoproteins (VLDLs) in the secretory apparatus of the hepatocyte relies on the mobilization of triacylglycerol (TAG) from the cytosolic pool by lipolysis and re-esterification. However, some of the re-esterified TAG products are returned to the cytosolic pool in the liver, which protects vulnerable body tissues from excess lipotoxic non-esterified fatty acids in the plasma. Some of the lipases involved in this process include arylacetamide deacetylase (AADAC) and its related proteins AADACL1 and AADACL2. AADAC, a single pass type II membrane protein of the endoplasmic reticulum, is expressed in hepatocytes, intestinal mucosal cells, pancreas and adrenal gland. It plays an important role in the metabolic activation of arylamine substrates to ultimate carcinogens. AADACL1 hydrolyzes the metabolic intermediate 2-acetyl monoalkylglycerol, and its inactivation results in disruption of ether lipid metabolism in cancer cells and impaired cell migration and tumor growth. |
| Gene ID | 13 |
|---|---|
| Other Names | CES5A1; DAC; 5033417E09Rik; Aada; AAAD_HUMAN; AADAC; 3.1.1.3; AAAD_MOUSE; AAAD_RAT; arylacetamide deacetylase; arylacetamide deacetylase (esterase) |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | AADAC (HGNC:17) |
|---|---|
| Function | Catalyzes the hydrolysis of ester and amide bonds and is largely involved in prodrugs activation, xenobiotic detoxification or toxicity, and, in some contexts, lipid and indole metabolism (PubMed:17936933, PubMed:18034159, PubMed:19339378, PubMed:2043131, PubMed:21856291, PubMed:22207054, PubMed:22415931, PubMed:23542347, PubMed:24125761, PubMed:24464802, PubMed:26164127, PubMed:27422753, PubMed:33446525, PubMed:34450168, PubMed:37002641, PubMed:8063807). Prefers compounds with relatively small acyl groups, especially compounds with acetyl group (PubMed:26164127). Participates in the hydrolysis of clinical drugs including acetyl esters compounds such as eslicarbazepine, abiraterone and rifamycins (rifampicin, rifabutin, and rifapentine) and N-acetylarylamine compounds such as flutamide, N- monoacetyldapsone, and phenacetin (PubMed:19339378, PubMed:21856291, PubMed:22207054, PubMed:22415931, PubMed:24125761, PubMed:24464802, PubMed:26164127, PubMed:27422753). Participates in the hydrolysis of carcinogen such as 2-acetylaminofluorene (AAF) and propanil (PubMed:2043131, PubMed:26164127, PubMed:8063807). In addition, plays a role in lipid metabolism by hydrolyzing newly synthesized diacylglycerol, so limiting triacylglycerol synthesis for cytoplasmic storage droplet formation and for VLDL assembly (PubMed:23542347). Can also catalyzes the deacetylation of cholesterol acetate (PubMed:18034159). Moreover can play a role in metabolism of pineal indoles by catalyzing the deacetylation of N-acetylarylalkylamine compounds including N-acetylserotonin (NAS), melatonin and N- acetyltryptamine, NAS conversion being the most efficient reaction (PubMed:37002641). |
| Cellular Location | Endoplasmic reticulum membrane; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q7M370}. Microsome membrane; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q7M370} |
| Tissue Location | Detected in liver (at protein level). Mainly expressed in liver, small intestine, colon, adrenal gland and bladder |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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Cat# AP99738
