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phospho-Ephrin B (Tyr329) Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB, IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | P98172 |
| Other Accession | P98172 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 38007 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr329 |
| Epitope Specificity | PV(p-Y)IV |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the ephrin family.Contains 1 ephrin RBD (ephrin receptor-binding) domain. |
| SUBUNIT | Interacts with GRIP1 and GRIP2. Binds to Nipah virus G protein. |
| Post-translational modifications | Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. |
| DISEASE | Defects in EFNB1 are a cause of craniofrontonasal syndrome (CFNS) [MIM:304110]; also known as craniofrontonasal dysplasia (CFND). CFNS is an X-linked inherited syndrome characterized by hypertelorism, coronal synostosis with brachycephaly, downslanting palpebral fissures, clefting of the nasal tip, joint anomalies, longitudinally grooved fingernails and other digital anomalies. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase. |
| Gene ID | 1947 |
|---|---|
| Other Names | CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2; Cek5-L; EFL-3; Epl2; LERK-2; Stra1; EFNB1_HUMAN; EFNB1; ELK ligand (ELK-L); EPH-related receptor tyrosine kinase ligand 2 (LERK-2); EFNB1_MOUSE; CEK5 receptor ligand (CEK5-L); Stimulated by retinoic acid gene 1 protein; ephrin B1; craniofrontonasal syndrome (craniofrontonasal dysplasia); ephrin-B1; EPH-related receptor tyrosine kinase ligand 2; EFNB2 | Ephrin B (phospho-Y329); p-Ephrin B; phospho-Ephrin B; p-EFNB2; EFNB2 | Ephrin B (phospho-Tyr329) |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | EFNB1 |
|---|---|
| Synonyms | EFL3, EPLG2, LERK2 |
| Function | Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development (PubMed:7973638, PubMed:8070404). Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells (PubMed:7973638, PubMed:8070404). Shows high affinity for the receptor tyrosine kinase EPHB1/ELK (PubMed:7973638, PubMed:8070404). Can also bind EPHB2 and EPHB3 (PubMed:8070404). Binds to, and induces collapse of, commissural axons/growth cones in vitro (By similarity). May play a role in constraining the orientation of longitudinally projecting axons (By similarity). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein. Membrane raft. Note=May recruit GRIP1 and GRIP2 to membrane raft domains [Ephrin-B1 intracellular domain]: Nucleus. Note=Colocalizes with ZHX2 in the nucleus. {ECO:0000250|UniProtKB:P52795} |
| Tissue Location | Widely expressed (PubMed:7973638, PubMed:8070404). Detected in both neuronal and non-neuronal tissues (PubMed:7973638, PubMed:8070404). Seems to have particularly strong expression in retina, sciatic nerve, heart and spinal cord (PubMed:7973638) |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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Cat# AP99865
