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HIPK3 Rabbit pAb
- 产品详情
- 实验流程
Application 
| IHC-P, IHC-F, IF, E |
|---|---|
| Primary Accession | Q9H422 |
| Other Accession | Q9H422 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 133743 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human HIPK3 |
| Epitope Specificity | 401-500/1215 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.Contains 1 protein kinase domain. |
| SUBUNIT | Interacts with Nkx1-2. Interacts with FAS and DAXX. Probably part of a complex consisting of HIPK3, FAS and FADD. Interacts with and stabilizes ligand-bound androgen receptor (AR). Interacts with UBL1/SUMO-1. Binds to NR5A1/SF1, SPEN/MINT and RUNX2. |
| Post-translational modifications | Autophosphorylated, but autophosphorylation is not required for catalytic activity.May be sumoylated. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. |
| Gene ID | 10114 |
|---|---|
| Other Names | DYRK6; FIST3; PKY; YAK1; mir-1902; ANPK; HIPK3_HUMAN; HIPK3; Androgen receptor-interacting nuclear protein kinase (ANPK); Fas-interacting serine/threonine-protein kinase (FIST); Homolog of protein kinase YAK1; 2.7.11.1; HIPK3_MOUSE; Nuclear body-associated kinase 3 (Nbak3); HIPK3_RAT; Fist; |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | HIPK3 |
|---|---|
| Synonyms | DYRK6, FIST3, PKY |
| Function | Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2- mediated activation of the osteocalcin FGF-responsive element (OCFRE). |
| Cellular Location | Cytoplasm. Nucleus |
| Tissue Location | Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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