癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
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细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
HIF 2 alpha Rabbit pAb
- 产品详情
- 实验流程
Application 
| WB |
|---|---|
| Primary Accession | P97481 |
| Other Accession | P97481 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 96712 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from mouse HIF 2 Alpha |
| Epitope Specificity | 1-130/874 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SIMILARITY | Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 2 PAS (PER-ARNT-SIM) domains. |
| SUBUNIT | Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBPB. |
| Post-translational modifications | In normoxia, is probably hydroxylated on Pro-405 and Pro-530 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. In normoxia, is hydroxylated on Asn-851 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. |
| Gene ID | 13819 |
|---|---|
| Other Names | ECYT4; HIF2A; HLF; MOP2; PASD2; bHLHe73; HIF-2alpha; HRF; EPAS; EPAS-1; HIF-2 alpha; HIF2 alpha; EPAS1_HUMAN; EPAS1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73 (bHLHe73); HIF-1-alpha-like factor (HLF); Hypoxia-inducible factor 2-alpha (HIF-2-alpha | HIF2-alpha); Member of PAS protein 2; PAS domain-containing protein 2; EPAS1_MOUSE; HIF-1-alpha-like factor (HLF | mHLF); HIF-related factor (HRF); EPAS1_RAT; endothelial PAS domain protein 1; HIF-1 alpha-like factor |
| Dilution | WB=1:500-2000,ICC/IF=1:50-200,Flow-Cyt=1ug/Test |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Epas1 |
|---|---|
| Synonyms | Hif2a |
| Function | Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity). |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981, ECO:0000269|PubMed:21546903}. Nucleus speckle. Note=Colocalizes with HIF3A isoform 2 in the nucleus and speckles. |
| Tissue Location | Expressed in most tissues, with highest levels in lung, followed by heart, kidney, brain and liver. Predominantly expressed in endothelial cells. Also found in smooth muscle cells of the uterus, neurons, and brown adipose tissue. High expression in embryonic choroid plexus and kidney glomeruli |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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