癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
PHH3
Rabbit Polyclonal Antibody (Mab)
- 产品详情
- 实验流程
Application 
| IHC |
|---|---|
| Primary Accession | Q92560 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 80362 Da |
| Gene ID | 8314 |
|---|---|
| Other Names | Ubiquitin carboxyl-terminal hydrolase BAP1, 3.4.19.12, BRCA1-associated protein 1, Cerebral protein 6 {ECO:0000303|Ref.2}, BAP1 {ECO:0000303|PubMed:9528852, ECO:0000312|HGNC:HGNC:950} |
| Dilution | IHC~~1:100~500 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PHH3 is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BAP1 {ECO:0000303|PubMed:9528852, ECO:0000312|HGNC:HGNC:950} |
|---|---|
| Function | Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed:12485996, PubMed:18757409, PubMed:20436459, PubMed:25451922, PubMed:35051358). Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed:20436459, PubMed:25451922, PubMed:30664650, PubMed:35051358). Does not deubiquitinate monoubiquitinated histone H2B (PubMed:20436459, PubMed:30664650). The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability (PubMed:20805357, PubMed:30664650, PubMed:36180891). Antagonizes PRC1 mediated H2AK119ub1 monoubiquitination (PubMed:30664650). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed:36180891). Recruited to specific gene-regulatory regions by YY1 (PubMed:20805357). Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N- terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed:19188440, PubMed:19815555). Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed:19188440, PubMed:19815555). Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed:19117993). It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed:19117993). Able to mediate autodeubiquitination via intramolecular interactions to counteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization (PubMed:34170818). |
| Cellular Location | Cytoplasm. Nucleus. Chromosome. Note=Mainly nuclear (PubMed:24703950, PubMed:30664650). Binds to chromatin (PubMed:30664650). Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin- protein ligase UBE2O (PubMed:24703950). Recruitment to chromatin is dependent on ASXL1/2/3 and recruitment to specific genes on FOXK1/2 (By similarity). Nuclear localization is redundantly mediated by the importin and transportin systems; TNPO1/transportin-1 is the major mediator of nuclear localization (PubMed:35446349) {ECO:0000250|UniProtKB:Q99PU7, ECO:0000269|PubMed:24703950, ECO:0000269|PubMed:30664650, ECO:0000269|PubMed:35446349} |
| Tissue Location | Highly expressed in testis, placenta and ovary (PubMed:9528852). Expressed in breast (PubMed:9528852). levels in the placenta increase over the course of pregnancy (PubMed:34170818) |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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