Anti-CTLA-4 / CD152 Reference Antibody (Antitope patent anti-CTLA4)

Recombinant Antibody

Anti-CTLA-4 / CD152 Reference Antibody (Antitope patent anti-CTLA4) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%
The purity of Anti-CTLA-4 / CD152 Reference Antibody (Antitope patent anti-CTLA4)is more than 95% ,determined by SEC-HPLC.
Immobilized human CTLA 4 His at 2 µg/mL can bind Anti-CTLA-4 / CD152 Reference Antibody (Antitope patent anti-CTLA4)，EC50=0.04907 µg/mL.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	P16410
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	24656 Da

Additional Information
Target/Specificity	CTLA-4 / CD152
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	CTLA4
Synonyms	CD152
Function	Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Cellular Location	Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalization
Tissue Location	Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.