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Anti-CDH1 / E-cadherin / CD324 Reference Antibody (Stem Centrx patent anti-Cadherin-1)

Recombinant Antibody

Anti-CDH1 / E-cadherin / CD324 Reference Antibody (Stem Centrx patent anti-Cadherin-1) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-CDH1 / E-cadherin / CD324 Reference Antibody (Stem Centrx patent anti-Cadherin-1)is more than 95% ,determined by SEC-HPLC.
Immobilized human CDH1 His at 2 µg/mL can bind Anti-CDH1 / E-cadherin / CD324 Reference Antibody (Stem Centrx patent anti-Cadherin-1)，EC50=0.004381 µg/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	P12830
Reactivity	Human, Mouse
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	97456 Da

Additional Information
Target/Specificity	CDH1 / E-cadherin / CD324
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	CDH1 (HGNC:1748)
Function	Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed:29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Cellular Location	Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein Endosome. Golgi apparatus, trans-Golgi network. Cytoplasm. Cell junction, desmosome. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. Recruited to desmosomes at the initial assembly phase and also accumulates progressively at mature desmosome cell-cell junctions (PubMed:25208567, PubMed:29999492) Localizes to cell-cell contacts as keratinocyte differentiation progresses (By similarity). {ECO:0000250\|UniProtKB:P09803, ECO:0000269\|PubMed:25208567, ECO:0000269\|PubMed:29999492}
Tissue Location	Expressed in granuloma macrophages (at protein level) (PubMed:27760340). Expressed in the skin (at protein level) (PubMed:22294297). Expressed in the liver (PubMed:3263290)