Anti-ADAM9 Reference Antibody (Imgc936)

Recombinant Antibody

Anti-ADAM9 Reference Antibody (Imgc936) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-ADAM9 Reference Antibody (Imgc936)is more than 98.27% ,determined by SEC-HPLC.
Immobilized Cyno ADAM9 His at 2 µg/mL can bind Anti-ADAM9 Reference Antibody (Imgc936)，EC50=0.003115 µg/mL.
Human ADAM9 CHOS cells were stained with Anti-ADAM9 Reference Antibody (Imgc936) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC70=0.3912 nM
The endocytosis ratio Imgc936 by Human ADAM9 HT29 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 50% at antibody concentration of 1 nM.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	Q13443
Reactivity	Mouse
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	90556 Da

Additional Information
Target/Specificity	ADAM9
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	ADAM9
Synonyms	KIAA0021, MCMP, MDC9, MLTNG
Function	Metalloprotease that cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as TEK, KDR, EPHB4, CD40, VCAM1 and CDH5. May mediate cell-cell, cell- matrix interactions and regulate the motility of cells via interactions with integrins.
Cellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein
Tissue Location	Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.