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Anti-CDH6 / K-Cadherin Reference Antibody (HKT288)

Recombinant Antibody

Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) is 97.93%, determined by SEC-HPLC.
Immobilized Cyno CDH6 His at 2 ug/mL can bind Anti-CDH6 / K-Cadherin Reference Antibody (HKT288), EC50=0.006944 ug/mL.
Human CDH6 HEK293 (A6) cells were stained with Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.08624 ug/mL.
The endocytosis ratio HKT288 by Hu-CDH6-HEK293(A6) increased with the increase of antibody concentration, and the Internalization Rate (%) reached 93% at antibody concentration of 2 nM.
HKT288 induced OVCAR3 Luciferase activity was evaluated using huCD16a(V158)-NF-AT-Jurkat Reporter Cell. The EC50 was approximately 0.6792 nM.
Mouse CDH6 / K-Cadherin HEK293 Cell Line were stained with HKT288 and negative control protein respectively, washed and then followed by PE and analyzed with FACS,EC50=0.1068 ug/mL.
Cynomolgus / Rhesus Macaque CDH6 / K-Cadherin HEK293 Cell Line were stained with HKT288 and negative control protein respectively, washed and then followed by PE and analyzed with FACS,EC50=0.1818 ug/mL.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P55285
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	88309 Da

Additional Information
Target/Specificity	CDH6 / K-Cadherin
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	CDH6
Function	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Highly expressed in brain, cerebellum, and kidney. Lung, pancreas, and gastric mucosa show a weak expression. Also expressed in certain liver and kidney carcinomas

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol