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Anti-TPBG Reference Antibody (PF-06263507)

Recombinant Antibody

Anti-TPBG Reference Antibody (PF-06263507) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-TPBG Reference Antibody (PF-06263507) is 99.25%, determined by SEC-HPLC.
Immobilized human 5T4 His at 2 ug/mL can bind Anti-TPBG Reference Antibody (PF-06263507), EC50=0.004604 ug/mL.
Human 5T4 HEK293 cells were stained with Anti-TPBG Reference Antibody (PF-06263507) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.7286 ug/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q13641
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	46032 Da

Additional Information
Target/Specificity	TPBG
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	TPBG
Synonyms	5T4
Function	May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol