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Anti-AA2AR / Adenosine A2aR Reference Antibody (3F6-9G5)

Recombinant Antibody

Anti-AA2AR / Adenosine A2aR Reference Antibody (3F6-9G5) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-AA2AR / Adenosine A2aR Reference Antibody (3F6-9G5) is 98.85%, determined by SEC-HPLC.
Human A2AR HEK293 Cell Line were stained with Anti-AA2AR / Adenosine A2aR Reference Antibody (3F6-9G5) and negative control protein respectively, washed and then followed by PE and analyzed with FACS
THP-1 were stained with Anti-AA2AR / Adenosine A2aR Reference Antibody (3F6-9G5) and negative control protein respectively, washed and then followed by PE and analyzed with FACS,The EC50 was approximately 0.2161 ug/mL.
Cynomolgus / Rhesus Macaque A2AR HEK293 Cell Line were stained with Anti-AA2AR / Adenosine A2aR Reference Antibody (3F6-9G5) and negative control protein respectively, washed and then followed by PE and analyzed with FACS,The EC50 was approximately 0.215 ug/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P29274
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	44707 Da

Additional Information
Target/Specificity	AA2AR / Adenosine A2aR
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	ADORA2A
Synonyms	ADORA2
Function	Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P30543}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P30543} Note=Colocalizes with GAS2L2 at neuronal processes {ECO:0000250\|UniProtKB:P30543}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol