Anti-LILRA4 / ILT7 / CD85g Reference Antibody (daxdilimab)

Recombinant Antibody

Anti-LILRA4 / ILT7 / CD85g Reference Antibody (daxdilimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-LILRA4 / ILT7 / CD85g Reference Antibody (daxdilimab)is more than 96.58% ,determined by SEC-HPLC.
Immobilized human ILT7 His at 2 µg/mL can bind Anti-LILRA4 / ILT7 / CD85g Reference Antibody (daxdilimab)，EC50=0.07254 µg/mL
Human ILT7 HEK293 cells were stained with Anti-LILRA4 / ILT7 / CD85g Reference Antibody (daxdilimab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC183=0.031 ug/mL

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	P59901
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	55181 Da

Additional Information
Target/Specificity	LILRA4 / ILT7 / CD85g
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	LILRA4 (HGNC:15503)
Function	Functions coreceptor to limit the innate immune responses to viral infections; signaling occurs via FCER1G (PubMed:16735691, PubMed:19564354). Down-regulates the production of IFNA1, IFNA2, IFNA4, IFNB1 and TNF by plasmacytoid dendritic cells that have been exposed to influenza virus or cytidine-phosphate-guanosine (CpG) dinucleotides, indicating it functions as a negative regulator of TLR7 and TLR9 signaling cascades (PubMed:16735691, PubMed:19564354, PubMed:24586760). Down-regulates interferon production in response to interaction with BST2 on HIV-1 infected cells (PubMed:26172439). Activates a signaling cascade in complex with FCER1G that results in phosphorylation of Src family and Syk kinases and thereby triggers mobilization of intracellular Ca(2+) (PubMed:16735691, PubMed:19564354). Does not interfere with the differentiation of plasmacytoid dendritic cells into antigen-presenting cells (PubMed:24586760).
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Detected on plasmacytoid dendritic cells (at protein level). Detected on plasmacytoid dendritic cells, but not on monocytes or B cells.