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Anti-BTN3A1 Reference Antibody (CTX-2026)

Recombinant Antibody

Anti-BTN3A1 Reference Antibody (CTX-2026) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-BTN3A1 Reference Antibody (CTX-2026) is 98.77%, determined by SEC-HPLC.
Immobilized human PVRIG His at 2 ug/mL can bind Anti-BTN3A1 Reference Antibody (CTX-2026), EC50=0.002394 ug/mL.
Human BTN3 HEK293 cells were stained with Anti-BTN3A1 Reference Antibody (CTX-2026) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.8254 nM.
Anti-BTN3A1 Reference Antibody（CTX-2026）captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human BTN3A1 / BTF5 / CD277, His Tag (Cat. PHA276) with an affinity constant of 9.89E-11M as determined in a SPR assay

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	O00481
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	57677 Da

Additional Information
Target/Specificity	BTN3A1
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	BTN3A1
Synonyms	BTF5
Function	Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Detected on T-cells, natural killer cells, dendritic cells and macrophages (at protein level). Ubiquitous. Highly expressed in heart, pancreas and lung, Moderately expressed in placenta, liver and muscle.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol