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Anti-CDH17 / Cadherin-17 Reference Antibody (10C12)

Recombinant Antibody

Anti- Human CDH17（ 10C12） on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of anti- Human CDH17（ 10C12） is 98.15%, determined by SEC-HPLC.
Immobilized human CDH17 (His Tag, 2 µg/mL) can bind to anti- Human CDH17（ 10C12） with an EC50 of 0.00468 ug/mL.
Human CDH17-expressing HEK293 cells were stained with Anti- Human CDH17（ 10C12） , with isotype and secondary-only controls. Flow analysis showed specific binding of the antibody, with an EC50 of 0.06889 ug/mL.
The endocytosis ratio of anti- human CDH17（ 10C12） in human CDH17-expressing HEK293 cells increased with rising antibody concentration, with the internalization rate reaching 80% at a concentration of 2 nM.
Anti- Human CDH17（ 10C12） inhibited the tumor growth of COLO205 on nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 70.0% at 5 mpk at D26.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q12864
Reactivity	Human
Clonality	Monoclonal
Isotype	Human IgG1
Calculated MW	92219 Da

Additional Information
Target/Specificity	CDH17 / Cadherin-17
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	CDH17
Function	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol