Anti-CDH17 / Cadherin-17 Reference Antibody (10C12)

Recombinant Antibody

Anti-CDH17 / Cadherin-17 Reference Antibody (10C12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-CDH17 / Cadherin-17 Reference Antibody (10C12)is more than 98.15% ,determined by SEC-HPLC.
Immobilized human CDH17, His Tag at 2 µg/mL can bind Anti-CDH17 / Cadherin-17 Reference Antibody (10C12)，EC50=0.00468 µg/mL
Human CDH17 HEK293 cells were stained with Anti-CDH17 / Cadherin-17 Reference Antibody (10C12) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC240=0.06889 µg/mL
The endocytosis ratio 10C12 by Human CDH17 HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 80% at antibody concentration of 2 nM.
10C12 inhibited the tumor growth of COLO205 on nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 70.0% at 5 mpk at D26.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	Q12864
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	92219 Da

Additional Information
Target/Specificity	CDH17 / Cadherin-17
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	CDH17
Function	Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.