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Anti-ROR1 Reference Antibody (zilovertamab vedotin)

Recombinant Antibody

Anti-ROR1 Reference Antibody (zilovertamab vedotin) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-ROR1 Reference Antibody (Zilovertamab vedotin) is 98.21%, determined by SEC-HPLC.
Immobilized human ROR1 His at 2 ug/mL can bind Anti-ROR1 Reference Antibody (Zilovertamab vedotin), The EC50 is 0.002833 ug/mL.
Anti-ROR1 Reference Antibody (Zilovertamab vedotin) inhibited the tumor growth of MDA-MB-231on NSG mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 96.6% at 10 mpk.
The endocytosis ratio Zilovertamab by Human ROR1-HEK293T cells increased with the increase of antibody concentration, and the Internalization Rate (%) reached 80% .
Human ROR1 HEK293T cells were stained with Anti-ROR1 Reference Antibody (Zilovertamab vedotin) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, The EC50 is 0.05656 ug/ml.
Human ROR1 CHO-K Cell Line were stained Anti-ROR1 Reference Antibody (zilovertamab vedotin) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, The EC50 is 0.03245 ug/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q01973
Reactivity	Human
Clonality	ADC
Isotype	IgG1
Calculated MW	104283 Da

Additional Information
Target/Specificity	ROR1
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	MMAE
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	ROR1
Synonyms	NTRKR1
Function	Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo (PubMed:25029443). Receptor for ligand WNT5A which activates downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling (PubMed:25029443, PubMed:27162350). In the inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells (PubMed:27162350). Via IGFBP5 ligand, forms a complex with ERBB2 to enhance CREB oncogenic signaling (PubMed:36949068).
Cellular Location	Cell membrane; Single-pass type I membrane protein. Cell projection, axon {ECO:0000250\|UniProtKB:Q9Z139}
Tissue Location	Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm