> 首页 > 产品 > 一抗 > 基因重组 > Anti-TREM1 / CD354 Reference Antibody (PY159)

Anti-TREM1 / CD354 Reference Antibody (PY159)

Recombinant Antibody

Anti-TREM1 / CD354 Reference Antibody (PY159) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-TREM1 / CD354 Reference Antibody (PY159) is more than 95%, determined by SEC-HPLC.
Immobilized human TREM1 His at 2 ug/mL can bind Anti-TREM1 / CD354 Reference Antibody (PY159), EC50=0.00645 ug/mL.
Human TREM1 HEK293 cells were stained with Anti-TREM1 / CD354 Reference Antibody (PY159) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.7744nM.
The endocytosis ratio PY159 by hu-TREM1-HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 80% at antibody concentration of 0.3 nM.
Anti-TREM1 Reference Antibody (PY159) Activation was evaluated using PBMC. The max induction fold was approximately 3.16

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	Q9NP99
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	26387 Da

Additional Information
Target/Specificity	TREM1 / CD354
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	TREM1
Function	[Isoform 1]: Cell surface receptor that plays important roles in innate and adaptive immunity by amplifying inflammatory responses (PubMed:10799849, PubMed:21393102). Upon activation by various ligands such as PGLYRP1, HMGB1 or HSP70, multimerizes and forms a complex with transmembrane adapter TYROBP/DAP12 (PubMed:17568691, PubMed:25595774, PubMed:29568119). In turn, initiates a SYK-mediated cascade of tyrosine phosphorylation, activating multiple downstream mediators such as BTK, MAPK1, MAPK3 or phospholipase C-gamma (PubMed:14656437, PubMed:21659545). This cascade promotes the neutrophil- and macrophage- mediated release of pro-inflammatory cytokines and/or chemokines, as well as their migration and thereby amplifies inflammatory responses that are triggered by bacterial and fungal infections (PubMed:17098818, PubMed:17568691). By also promoting the amplification of inflammatory signals that are initially triggered by Toll-like receptor (TLR) and NOD-like receptor engagement, plays a major role in the pathophysiology of acute and chronic inflammatory diseases of different etiologies including septic shock and atherosclerosis (PubMed:11323674, PubMed:21393102).
Cellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein. Note=Recruited to lipid rafts when activated.
Tissue Location	Mostly expressed by immune cells of the myeloid lineage, such as monocytes, macrophages, neutrophils and dendritic cells (PubMed:10799849). Expression is associated with a mature stage of myeloid development (PubMed:11922939). Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues Isoform 2 was detected in the lung, liver and mature monocytes