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Anti-NRP1 / VEGF165R / CD304 Reference Antibody (vesencumab)

Recombinant Antibody

Anti-NRP1 / VEGF165R / CD304 Reference Antibody (vesencumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-NRP1 / VEGF165R / CD304 Reference Antibody (vesencumab)is more than 100% ,determined by SEC-HPLC.
Immobilized human NRP1 His at 2 µg/mL can bind Anti-NRP1 / VEGF165R / CD304 Reference Antibody (vesencumab)，EC50=0.005571 µg/mL
Human NRP1 HEK293 cells were stained with Anti-NRP1 / VEGF165R / CD304 Reference Antibody (vesencumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC302=0.1948 µg/mL

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	O14786
Reactivity	Human, Mouse
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	103134 Da

Additional Information
Target/Specificity	NRP1 / VEGF165R / CD304
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	NRP1 (HGNC:8004)
Synonyms	NRP, VEGF165R
Function	Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed:10688880, PubMed:9288753, PubMed:9529250). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed:19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed:10688880, PubMed:19805273, PubMed:9288753, PubMed:9529250). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed:30623799).
Cellular Location	[Isoform 2]: Secreted
Tissue Location	[Isoform 1]: The expression of isoforms 1 and 2 does not seem to overlap. Expressed in olfactory epithelium (at protein level) (PubMed:33082293). Expressed in fibroblasts (at protein level) (PubMed:36213313). Expressed by the blood vessels of different tissues In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain (PubMed:10688880, PubMed:9529250). Expressed in the central nervous system, including olfactory related regions such as the olfactory tubercles and paraolfactory gyri (PubMed:33082293)