Anti-B7-H5 / VISTA Reference Antibody (onvatilimab)

Recombinant Antibody

Anti-B7-H5 / VISTA Reference Antibody (onvatilimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%
The purity of Anti-B7-H5 / VISTA Reference Antibody (onvatilimab)is more than 98.11% ,determined by SEC-HPLC.
Immobilized human B7-H5 / VISTA His at 2 µg/mL can bind Anti-B7-H5 / VISTA Reference Antibody (onvatilimab)，EC50=0.01513 µg/mL
Human B7H5 CHO-K cells were stained with Anti-B7-H5 / VISTA Reference Antibody (onvatilimab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC328=0.9471 µg/mL

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, Kinetics, Animal Model
Primary Accession	Q9H7M9
Reactivity	Human, Mouse
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	33908 Da

Additional Information
Target/Specificity	B7-H5 / VISTA
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO Cell
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

Protein Information
Name	VSIR (HGNC:30085)
Function	Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14- mediated MMP2 activation (PubMed:20666777).
Cellular Location	Cell membrane; Single-pass type I membrane protein
Tissue Location	Expressed in spleen. Detected on a number of myeloid cells including CD11b monocytes, CD66b+ neutrophils, at low levels on CD4+ and CD8+ T-cells, and in a subset of NK cells. Not detected on B cells (at protein level). Expressed at high levels in placenta, spleen, plasma blood leukocytes, and lung. Expressed at moderate levels in lymph node, bone marrow, fat, uterus, and trachea Has low expression levels in other tissues

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.