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Anti-IL-7Ra / CD127 Reference Antibody (PF-06342674)

Recombinant Antibody

Anti-IL-7Ra / CD127 Reference Antibody (PF-06342674) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-IL-7Ra / CD127 Reference Antibody (PF-06342674) is 99.69%, determined by SEC-HPLC.
Immobilized Cynomolgus / Rhesus Macaque IL-7Ra / CD127, His Tag at 2 µg/mL can bind Anti-IL-7Ra / CD127 Reference Antibody (PF-06342674), The EC50 was approximately 0.02866 ug/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P16871
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	51579 Da

Additional Information
Target/Specificity	IL-7Ra / CD127
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	IL7R
Function	Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Cellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein [Isoform 4]: Secreted.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol