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Anti-IL-2Ra / CD25 Reference Antibody (basiliximab)

Recombinant Antibody

Anti-IL-2Ra / CD25 Reference Antibody (basiliximab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 90%.
The purity of Anti-IL-2Ra / CD25 Reference Antibody (basiliximab) is more than 95%, determined by SEC-HPLC.
Immobilized Human IL-2Ra / CD25, His Tag Protein at 2 ug/mL can bind Anti-IL-2Ra / CD25 Reference Antibody (basiliximab)，EC50=0.006825 ug/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P01589
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	30819 Da

Additional Information
Target/Specificity	IL-2Ra / CD25
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	IL2RA
Function	Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Cellular Location	Membrane; Single-pass type I membrane protein.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol