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Anti-Spike RBD Reference Antibody (Romlusevimab)

Recombinant Antibody

Anti-Spike RBD Reference Antibody (Romlusevimab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-Spike RBD Reference Antibody (Romlusevimab) is 98.1%, determined by SEC-HPLC.
Immobilized B1-617-2-India-hFc at 2 µg/mL can bind Romlusevimab, EC50= 0.02520 µg/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P0DTC2
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG1
Calculated MW	141178 Da

Additional Information
Target/Specificity	Spike RBD
Endotoxin	< 0.001EU/ µg,determined by LAL method.
Conjugation	Unconjugated
Expression system	CHO
Format	Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	S {ECO:0000255\|HAMAP-Rule:MF_04099}
Function	[Spike protein S1]: Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2 (PubMed:32142651, PubMed:32155444, PubMed:33607086). When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane (PubMed:34561887). When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane (PubMed:32075877, PubMed:32221306, PubMed:34903715, PubMed:36779763). Alternatively, may use NRP1/NRP2 (PubMed:33082294, PubMed:33082293) and integrin as entry receptors (PubMed:35150743). The use of NRP1/NRP2 receptors may explain the tropism of the virus in human olfactory epithelial cells, which express these molecules at high levels but ACE2 at low levels (PubMed:33082293). Uses also ASGR1 as an alternative receptor in an ACE2-independent manner (PubMed:34837059). The stalk domain of S contains three hinges, giving the head unexpected orientational freedom (PubMed:32817270).
Cellular Location	Virion membrane {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:32979942}; Single-pass type I membrane protein {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:34504087}. Host endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:34504087}; Single- pass type I membrane protein {ECO:0000255\|HAMAP-Rule:MF_04099}. Host cell membrane {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:34504087}; Single-pass type I membrane protein {ECO:0000255\|HAMAP-Rule:MF_04099}. Note=Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion (PubMed:34504087). An average of 26 +/-15 S trimers are found randomly distributed at the surface of the virion (PubMed:32979942) {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:32979942, ECO:0000269\|PubMed:34504087}