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Anti-Spike S1 Reference Antibody (CR3022)

Anti-Spike S1 Reference Antibody (CR3022) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The purity of Anti-Spike S1 Reference Antibody (CR3022) is 99.5%, determined by SEC-HPLC.
Immobilized SARS-CoV 2 Spike RBD (B1.617.2-India), Fc Tag at 2 ug/mL can bind CR3022, The EC50 is 0.02631 µg/mL.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	Functional assay, Kinetics (SPR), Kinetics (BLI), FACS, E, FTA
Primary Accession	P59594
Reactivity	Human
Clonality	Monoclonal
Isotype	IgG
Calculated MW	139125 Da

Additional Information
Target/Specificity	Spike S1
Expression system	CHO

For Research Use Only. Not For Use In Diagnostic Procedures.

Patent Notice: The variable region sequence of this antibody is derived from a published third-party patent. This product is manufactured independently as a Research Use Only (RUO) reagent and is not licensed, sponsored, or endorsed by the patent holder. It is intended solely for research purposes. Customers considering any commercial, diagnostic, or therapeutic use should independently evaluate freedom-to-operate with respect to relevant third-party patents.

Protein Information
Name	S {ECO:0000255\|HAMAP-Rule:MF_04099}
Function	[Spike glycoprotein]: May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.
Cellular Location	Virion membrane {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:15831954}; Single-pass type I membrane protein {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:15831954}. Host endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:20861307}; Single- pass type I membrane protein {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:15831954}. Host cell membrane {ECO:0000255\|HAMAP- Rule:MF_04099, ECO:0000269\|PubMed:15831954}; Single-pass type I membrane protein {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:15831954}. Note=Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment (PubMed:20861307). Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion. {ECO:0000255\|HAMAP-Rule:MF_04099, ECO:0000269\|PubMed:20861307}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol